HPLC Mobile Phase (Isocratic)
Calculate total solvent volume based on Run Time and Overage
INPUTS
mL/min
min
Inj.
%
OUTPUT
Minimum Volume Required:
0 mL
Volume to Prepare (w/ Overage):
0 mL
Calculated By:
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How to Use the HPLC Mobile Phase Calculator
Proper planning of mobile phase preparation is essential in High-Performance Liquid Chromatography (HPLC) to prevent running dry during a sequence, which can damage the column and pump seals.
Step 1: Enter Method Parameters
Input the Flow Rate (mL/min) and the total Runtime (minutes per injection) as defined in your analytical method (STP).
Step 2: Enter Sequence Details
Enter the total Number of Injections. This should include: System Suitability, Standards, Samples, and Bracketing Standards.
Step 3: Add Safety Overage
It is GMP best practice to prepare excess mobile phase to account for evaporation, priming, and potential re-injections. A standard overage is between 10% to 20%.
Key Calculation Formula
Total Vol = (Flow × Time × Inj) × (1 + %Overage/100)