HPLC ↔ UPLC Method Transfer
Scale Flow Rate, Injection Volume & Gradient Table (Geometric Scaling)
Original Method (Source)
Transferred Method (Target)
| Original Time (min) | % A | % B | % C | Target Time (min) |
|---|---|---|---|---|
User Guide: Method Transfer Calculator
Use this tool to geometrically scale analytical methods from conventional HPLC to UPLC (or vice versa), maintaining separation quality while optimizing speed.
1. Input Source Method Parameters
Enter the column details ($L$, $dc$, $dp$), Flow Rate, and Injection Volume of your current validated method.
2. Input Target Method Parameters
Enter the dimensions of the new column (e.g., UPLC 1.7 µm column). The calculator will instantly preview the scaled Flow Rate and Injection Volume.
3. Dwell Volume (System Delay)
Enter the Dwell Volume ($V_d$) for both systems if known. This allows the calculator to adjust the gradient timing to ensure the solvent composition reaches the column at the correct relative time.
4. Gradient Table
Enter your original gradient time program and composition (%A, %B, %C). Clicking Transfer Method will calculate the new time points for the target column.